EPO Purification

Easy-to-use EPO Purification kits from MAIIA Diagnostics offer a unique system for rapid immuno-affinity purification and concentration of erythropoietins (EPO) from aqueous media such as urine, serum, plasma, dried blood spots or cellculture medium.

The anti-EPO column in the kits is immobilized with anti-EPO antibody which captures both endogenous erythropoietin (hEPO) and recombinant erythropoietin (rhEPO) such as Epoetins, NESP, CERA and EPO-Fc. The kits are intended to be used as a preanalytical sample preparation step for further analysis with techniques such as isoelectric focusing (IEF), SARCOSYL polyacrylamide gel electrophoresis (SAR-PAGE) and Membrane Assisted Isoform ImmunoAssay (MAIIA). Several purifications can simultaneously be handled if using appropriate laboratory equipment.

EPO Purification Kit (Art.1390)

25 disposable anti-EPO columns and buffers included in the kit. Suitable for few purifications per day.

Sample capacity:

10–20 mL urine or 0.5–1.0 mL serum / plasma

Purification:

Buffers are added to sample and after filtration the sample mixture is transferred to a membrane based Anti EPO column with immobilized anti-EPO antibodies for pass through purification. The bound EPO in the membrane is then released with elution buffer and spin off by using a micro centrifuge.

Purified sample:

35 – 55 µL

Analysis:

IEF, SAR-PAGE, MAIIA

Accessories:

Funnel Pack F40 (25 pcs, Art.1340). Order separately.

Equipments:

0.45 μm HPF Millex HV filter, 50 mL syringe with Luer-Lok, vacuum manifold with Luer female taper connection, vacuum source with regulator to provide a steady vacuum and microcentrifuge.

Downloads:

DfU MSDS

EPO Purification Gel Kit for Urine (Art.1410) or for Blood (Art.1430)

25 disposable anti-EPO gel columns and buffers included in the kit. Suitable for running many purifications simultaneously, typical 100 or more per day.

Sample capacity:

10 mL urine (Art. 1410)
0.5 mL serum/plasma or 1-3 dried blood spot discs (Art. 1430)

Purification:

Buffers are added to sample and after filtration the sample mixture is transferred to anti-EPO resin for end over end incubation for 2 hours. Aqueous media is removed by pressure format and the bound EPO in the resin is then released with elution buffer and spin off by using a micro centrifuge.

Purified sample:

50 µL

Analysis:

SAR-PAGE

Accessories:

Funnel Pack F20 (25 pcs, Art.1420). Order separately.

Equipments:

0.45 μm HPF Millex HV filter, 20 mL syringe with Luer-Lok, vacuum manifold with Luer female taper connection, vacuum source with regulator, microcentrifuge and tube rotator for 50 mL tube.

Downloads:

DfU (Art.1410) MSDS (Art.1410) DfU (Art.1430) MSDS (Art.1430)

EPO Purification Gel (Art.1271)

One regenerable high capacity anti-EPO gel column and buffers for research used. Custom quantities 2-10 mL are available.

Sample capacity:

For larger sample volumes or high EPO concentrations such as cellculture medium. Bindingcapacity 100 – 400 µg EPO / 2 mL gel depending on EPO variant.

Purification:

Sample passes through a traditional regenerable anti-EPO gel column by gravity format. Flow rate 0.5 – 3mL / min. The bound EPO is then released with elution buffer in the same way.

Purified sample:

3 – 5 mL

Analysis:

IEF, SAR-PAGE, MAIIA

Accessories:

Equipments:

No extra equipments needed.

Downloads:

DfU MSDS

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