EPO Purification
Easy-to-use EPO Purification kits from MAIIA Diagnostics offer a unique system for rapid immuno-affinity purification and concentration of erythropoietins (EPO) from aqueous media such as urine, serum, plasma, dried blood spots or cellculture medium.
The anti-EPO column in the kits is immobilized with anti-EPO antibody which captures both endogenous erythropoietin (hEPO) and recombinant erythropoietin (rhEPO) such as Epoetins, NESP, CERA and EPO-Fc. The kits are intended to be used as a preanalytical sample preparation step for further analysis with techniques such as isoelectric focusing (IEF), SARCOSYL polyacrylamide gel electrophoresis (SAR-PAGE) and Membrane Assisted Isoform ImmunoAssay (MAIIA). Several purifications can simultaneously be handled if using appropriate laboratory equipment.
EPO Purification Kit (Art.1390)
Sample capacity:
10–20 mL urine or 0.5–1.0 mL serum / plasma
Purification:
Buffers are added to sample and after filtration the sample mixture is transferred to a membrane based Anti EPO column with immobilized anti-EPO antibodies for pass through purification. The bound EPO in the membrane is then released with elution buffer and spin off by using a micro centrifuge.
Purified sample:
Analysis:
Accessories:
Equipments:
0.45 μm HPF Millex HV filter, 50 mL syringe with Luer-Lok, vacuum manifold with Luer female taper connection, vacuum source with regulator to provide a steady vacuum and microcentrifuge.
Downloads:
EPO Purification Gel Kit for Urine (Art.1410)
EPO Purification Gel Kit for Blood (Art.1430)
Sample capacity:
10 mL urine (Art. 1410)
0.5 mL serum/plasma or 1-3 dried blood spot discs (Art. 1430)
Purification:
Buffers are added to sample and after filtration the sample mixture is transferred to anti-EPO resin for end over end incubation for 2 hours. Aqueous media is removed by pressure format and the bound EPO in the resin is then released with elution buffer and spin off by using a micro centrifuge.
Purified sample:
50 µL
Analysis:
Accessories:
Equipments:
0.45 μm HPF Millex HV filter, 20 mL syringe with Luer-Lok, vacuum manifold with Luer female taper connection, vacuum source with regulator, microcentrifuge and tube rotator for 50 mL tube.
Downloads:
EPO Purification Gel (Art.1271)
Sample capacity:
Purification:
Sample passes through a traditional regenerable anti-EPO gel column by gravity format. Flow rate 0.5 – 3mL / min. The bound EPO is then released with elution buffer in the same way.
Purified sample:
Analysis:
Accessories:
Equipments:
Downloads:
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