Research
Are you working with human, mouse or monkey models in your EPO research? We can provide the tools to you.
Purify
Quantify
Analyse isoforms
Purification of EPO removes the major proportion of other proteins and can also concentrate your sample. Our purification tools can be used to purify EPO from different biological fluids, including DBS samples, as well as from cell culture media.
Quantification of the total amount of EPO can be done in some biological fluids directly or after purification. Our EPO Semi Quantification kit is in easy-to-use dipstick format.
Posttranslational modifications of a protein, such as glycosylation, can significantly impact its biological functions. Differences in the EPO isoform pattern may occur between EPO produced in different cell types, such as liver or kidney cells, between normal cells and tumour cells, or between human cells and cells from other species, such as many recombinant EPO variants. Our isoform test is built on a dipstick, combining affinity chromatography and EPO immuno assay.
Do not hesitate to contact us for more info!
Research articles from some of our customers:
** EPO Purification Gel kit 3F6 (art no. 1410, 1430) or 7D3 (art no. 1460)
Author(s): D Schwenke, et al. doi: 10.1002/dta.3821 (2024)
Author(s): Heiland C, et al. doi:10.1002/dta.3596 (2023)
Author(s): Requena-Tutusaus L, et al. doi: 10.4155/bio-2023-0118 (2023)
Author(s): Kobayashi H, et al. doi: 10.1111/apha.13826 (2022)
Author(s): Heiland C, et al. doi: 10.1002/dta.3260 (2022)
Author(s): Heiland C, et al. doi:10.1002/dta.2730 (2019)
** EPO Purification Kit 3F6 (art no. 1390)
Author(s): Postnikov P, et al. doi:10.1002/dta.1916 (2015)
Author(s): Ludovic Bailly-Chouriberry, et al. doi:10.1039/C2AN15662H (2012).
Author(s): Lönnberg M, et al. doi:10.1016/j.chroma.2010.09.034. (2010).
Author(s): Dehnes Y, et al. doi:10.1016/j.jpba.2010.06.017. (2010).
** Membrane-Assisted Isoform ImmunoAssay (MAIIA)
Author(s): Lönnberg M, et al. doi:10.1016/j.ab.2011.09.021 (2012).
Author(s): Lönnberg M, et al. doi:10.1007/s00216-012-5972-0 (2012).
Author(s): J. Mørkeberg K, et al. doi:10.1111/sms.12049 (2014)
Author(s): Lönnberg M, et al. doi:10.1016/j.jim.2008.09.022 (2008).
Author(s): L Franco Fraguas, et al. doi:10.1016/j.chroma.2008.10.036. (2008).
Author(s): de Seigneux S, et al. doi:10.1681/ASN.2015050508 (2016)
.Author(s): Aachmann-Andersen N.J, et al. doi:10.1371/journal.pone.0110903 (2014)
Author(s): Lönnberg M
Doctoral thesis, ISBN: 91-554-5250-7, Uppsala University, Sweden (2002).
Author(s): Lönnberg M, et al. doi:10.1016/S0378-4347(01)00376-0. (2001).
Author(s): Lundby AK, et al. doi:10.1007/s00421-014-2844-7 (2014)
Author(s): Lönnberg M, et al. doi:10.1016/S0022-1759(00)00287-8. (2000).
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