Buffer for Urine (Discontinued!)
Precipitates of different colours and shapes are commonly appearing in urine specimens, especially in frozen and thawed samples. The precipitates may bind large amounts of substances, especially proteins, occurring in the urine. The elimination of precipitates, using centrifugation or filtration, should be avoided as this also may remove urine components to be analysed. Buffer for Urine (Art. No. 1400) rapidly dissolves precipitates appearing in thawed urine specimens.
It has e.g. been found that up to 85% of the glycoprotein hormone erythropoietin in urine is bound to the precipitates, but can be released by the use of Buffer for urine [Lönnberg et al 2008]. This urine mixture has been used for EPO lateral flow test analysis, after desalting, or before affinity purification of urine EPO.
The Buffer for urine might also be valuable for the release of several other proteins and low molecular weight substances thus making them available for analysis or further purification. A volume of 1 mL UPD is sufficient for 10 mL urine.
Lönnberg, M., Drevin, M., Carlsson, J. Journal of Immunological Methods 339(2), 236-244 (2008) Ultra-sensitive immunochromatographic assay for quantitative determination of erythropoietin.