Easy-to-use EPO Purification kits from MAIIA Diagnostics offer a unique system for rapid immuno-affinity purification and concentration of erythropoietins (EPO) from aqueous media such as urine, serum, plasma or cellculture medium.
The anti-EPO column in the kits is immobilized with anti-EPO antibody which captures both endogenous erythropoietin (hEPO) and recombinant erythropoietin (rhEPO) such as Epoetins, NESP, CERA and EPO-Fc. The kits are intended to be used as a preanalytical sample preparation step for further analysis with techniques such as isoelectric focusing (IEF), SARCOSYL polyacrylamide gel electrophoresis (SAR-PAGE) and Membrane Assisted Isoform ImmunoAssay (MAIIA). Several purifications can simultaneously be handled if using appropriate laboratory equipment.
|EPO Purification Kit (Art.1390)||EPO Purification Gel Kit for Urine (Art.1410)
EPO Purification Gel Kit for Blood (Art.1430)
Available in June 2019
|EPO Purification Gel (Art.1271)|
|Description||25 disposable anti-EPO columns and buffers included in the kit. Suitable for few purifications per day.||25 disposable anti-EPO gel columns and buffers included in the kit. Suitable for running many purifications simultaneously, typical 100 or more per day.||Regenerable high capacity anti-EPO gel column and buffers for research used. Custom quantities 2-10 mL are available.|
|Sample||10 – 20 mL urine or
0.5 – 1.0 mL serum / plasma
|10 mL urine
0.5 mL serum or dried blood spot
|For larger sample volumes or high EPO concentrations such as cellculture medium. Bindingcapacity 100 – 400 µg EPO / 2 mL gel depending on EPO variant.|
|Purification||Sample passes through a filter with immobilized anti-EPO antibodies by pressure format. Flow rate 1mL / min. Bound EPO is then released using a micro centrifuge.||Sample incubates with anti-EPO affinity resin by end over end incubation for 60 – 90 min. Aqueous media is then removed by pressure format. Bound EPO is then released a using a micro centrifuge.||Sample passes through a traditional regenerable anti-EPO gel column by gravity format. Flow rate 0,5 – 3mL / min.|
|Purified sample||35 – 55 µL||35- 50 µL||3 – 5 mL|
|Analysis||IEF, SAR-PAGE, MAIIA||SAR-PAGE||IEF, SAR-PAGE, MAIIA|
|Accessories||Funnel Pack F40 (25 pcs, Art.1340).
|Funnel Pack F20 (25 pcs, Art.1420).
|Equipments||0.45 μm HPF Millex HV filter and 50 mL syringe with Luer-Lok, vacuum manifold with Luer female taper connection, vacuum source and a regulator to provide a steady vacuum or similar. Microcentrifuge. Tube rotator (for art.1410 and Art.1430).||No extra equipments needed.|
|DFU (Art.1410, Art.1430)