Easy-to-use EPO Purification kits from MAIIA Diagnostics offer a unique system for rapid immuno-affinity purification and concentration of erythropoietins (EPO) from aqueous media such as urine, serum, plasma or cellculture medium.
The anti-EPO column in the kits is immobilized with anti-EPO antibody which captures both endogenous erythropoietin (hEPO) and recombinant erythropoietin (rhEPO) such as Epoetins, NESP, CERA and EPO-Fc. The kits are intended to be used as a preanalytical sample preparation step for further analysis with techniques such as isoelectric focusing (IEF), SARCOSYL polyacrylamide gel electrophoresis (SAR-PAGE) and Membrane Assisted Isoform ImmunoAssay (MAIIA). Several purifications can simultaneously be handled if using appropriate laboratory equipment.
| EPO Purification Kit (Art.1390) | EPO Purification Gel Kit for Urine (Art.1410) EPO Purification Gel Kit for Blood (Art.1430) |
EPO Purification Gel (Art.1271) | |
| Description | 25 disposable anti-EPO columns and buffers included in the kit. Suitable for few purifications per day. | 25 disposable anti-EPO gel columns and buffers included in the kit. Suitable for running many purifications simultaneously, typical 100 or more per day. | Regenerable high capacity anti-EPO gel column and buffers for research used. Custom quantities 2-10 mL are available. |
| Sample | 10 – 20 mL urine or 0.5 – 1.0 mL serum / plasma |
(Art. 1410) 10 mL urine (Art. 1430) 0.5 mL serum/plasma or 1-2 dried blood spot discs |
For larger sample volumes or high EPO concentrations such as cellculture medium. Bindingcapacity 100 – 400 µg EPO / 2 mL gel depending on EPO variant. |
| Purification | Sample passes through a membrane with immobilized anti-EPO antibodies by pressure format. Flow rate 1mL / min. The bound EPO in the membrane is then released with elution buffer and spin off by using a micro centrifuge. | Sample incubates with anti-EPO resin by end over end incubation for 60 – 90 min. Aqueous media is then removed by pressure format. The bound EPO in the resin is then released with elution buffer and spin off by using a micro centrifuge. | Sample passes through a traditional regenerable anti-EPO gel column by gravity format. Flow rate 0,5 – 3mL / min. The bound EPO is then released with elution buffer in the same way. |
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| Purified sample | 35 – 55 µL | (Art. 1410) 50 µL (Art. 1430) 35 µL |
3 – 5 mL |
| Analysis | IEF, SAR-PAGE, MAIIA | SAR-PAGE | IEF, SAR-PAGE, MAIIA |
| Accessories | Funnel Pack F40 (25 pcs, Art.1340). Order separately. |
Funnel Pack F20 (25 pcs, Art.1420). Order separately. |
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| Equipments | 0.45 μm HPF Millex HV filter and 50 mL syringe with Luer-Lok, vacuum manifold with Luer female taper connection, vacuum source and a regulator to provide a steady vacuum or similar. Microcentrifuge. Tube rotator (for art.1410 and Art.1430).
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No extra equipments needed. | |
| Downloads | DFU MSDS |
DFU (Art.1410, Art.1430) MSDS |
DFU MSDS |
