EPO Purification

Easy-to-use EPO Purification kits from MAIIA Diagnostics offer a unique system for rapid immuno-affinity purification and concentration of erythropoietins (EPO) from aqueous media such as urine, serum, plasma or cellculture medium.

The anti-EPO column in the kits is immobilized with anti-EPO antibody which captures both endogenous erythropoietin (hEPO) and recombinant erythropoietin (rhEPO) such as Epoetins, NESP, CERA and EPO-Fc. The kits are intended to be used as a preanalytical sample preparation step for further analysis with techniques such as isoelectric focusing (IEF), SARCOSYL polyacrylamide gel electrophoresis (SAR-PAGE) and Membrane Assisted Isoform ImmunoAssay (MAIIA). Several purifications can simultaneously be handled if using appropriate laboratory equipment.

 

EPO Purification Kit (Art.1390) EPO Purification Gel Kit for Urine (Art.1410)
EPO Purification Gel Kit for Blood (Art.1430)
EPO Purification Gel (Art.1271)
Description   25 disposable anti-EPO columns and buffers included in the kit. Suitable for few purifications per day. 25 disposable anti-EPO gel columns and buffers included in the kit. Suitable for running many purifications simultaneously, typical 100 or more per day. Regenerable high capacity anti-EPO gel column and buffers for research used. Custom quantities 2-10 mL are available.
Sample 10 – 20 mL urine or
0.5 – 1.0 mL serum / plasma
(Art. 1410) 10 mL urine
(Art. 1430) 0.5 mL serum/plasma or 1-2 dried blood spot discs
For larger sample volumes or high EPO concentrations such as cellculture medium. Bindingcapacity 100 – 400 µg EPO / 2 mL gel depending on EPO variant.
Purification  Sample passes through a membrane with immobilized anti-EPO antibodies by pressure format. Flow rate 1mL / min. The bound EPO in the membrane is then released with elution buffer and spin off by using a micro centrifuge. Sample incubates with anti-EPO resin by end over end incubation for 60 – 90 min. Aqueous media is then removed by pressure format. The bound EPO in the resin is then released with elution buffer and spin off by using a micro centrifuge. Sample passes through a traditional regenerable anti-EPO gel column by gravity format. Flow rate 0,5 – 3mL / min. The bound EPO is then released with elution buffer in the same way.
                     
Purified sample 35 – 55 µL (Art. 1410) 50 µL
(Art. 1430) 35 µL
3 – 5 mL
Analysis IEF, SAR-PAGE, MAIIA SAR-PAGE IEF, SAR-PAGE, MAIIA
Accessories  Funnel Pack F40 (25 pcs, Art.1340).
Order separately.
Funnel Pack F20 (25 pcs, Art.1420).
Order separately.
Equipments  0.45 μm HPF Millex HV filter and 50 mL syringe with Luer-Lok, vacuum manifold with Luer female taper connection, vacuum source and a regulator to provide a steady vacuum or similar. Microcentrifuge. Tube rotator (for art.1410 and Art.1430).

No extra equipments needed.
Downloads DFU
MSDS
DFU (Art.1410, Art.1430)
MSDS
DFU
MSDS