EPO Purification

Easy-to-use EPO Purification kits from MAIIA Diagnostics offer a unique system for rapid immuno-affinity purification and concentration of erythropoietins (EPO) from aqueous media such as urine, serum, plasma or cellculture medium.

The anti-EPO column in the kits is immobilized with anti-EPO antibody which captures both endogenous erythropoietin (hEPO) and recombinant erythropoietin (rhEPO) such as Epoetins, NESP, CERA and EPO-Fc. The kits are intended to be used as a preanalytical sample preparation step for further analysis with techniques such as isoelectric focusing (IEF), SARCOSYL polyacrylamide gel electrophoresis (SAR-PAGE) and Membrane Assisted Isoform ImmunoAssay (MAIIA). Several purifications can simultaneously be handled if using appropriate laboratory equipment.

 

EPO Purification Kit
(Art. No. 1390)
EPO Purification Gel Kit
(Art. No. 1410)
Available in June 2019
EPO Purification Gel, 2 mL
(Art. No. 1271)
Description   25 disposable anti-EPO columns and buffers included in the kit. Suitable for few purifications per day. 25 disposable anti-EPO gel columns and buffers included in the kit. Suitable for running many purifications simultaneously, typical 100 or more per day. 1 regenerable 2 mL high capacity anti-EPO gel column and buffers. Custom quantities are available.
Sample 10 – 20 mL urine or
0.5 – 1.0 mL serum / plasma
10 mL urine Larger sample volumes or high EPO concentrations such as cellculture medium. Bindingcapacity 100 – 400 µg EPO depending on EPO variant.
Purification  Sample passes through a filter with immobilized anti-EPO antibodies by pressure format. Flow rate 1mL / min. Bound EPO is then released using a micro centrifuge. Sample incubates with anti-EPO affinity resin by end over end incubation for 90 min. Aqueous media is then removed by pressure format. Bound EPO is then released a using a micro centrifuge. Sample passes through a traditional regenerable anti-EPO gel column by gravity format. Flow rate 0,5 – 3mL / min.
                     
Purified sample 35 – 55 µL 50 µL 3 – 5 mL
Analysis IEF, SAR-PAGE, MAIIA SAR-PAGE IEF, SAR-PAGE, MAIIA
Accessories  Funnel Pack F40 (25 pcs, Art. No. 1340).
Order separately.
Funnel Pack F20 (25 pcs, Art. No. 1320).
Order separately.
EPO Purification Gel Running Buffers (Art. No. 1300). Order separately for rerun.
Equipments  0.45 μm HPF Millex HV filter and 50 mL syringe with Luer-Lok, vacuum manifold with Luer female taper connection, vacuum source and a regulator to provide a steady vacuum or similar. Microcentrifuge. Tube rotator (for art no 1410).

No extra equipments needed.
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